|
KMID : 0665220190320020148
|
|
Korean Journal of Food and Nutrition
2019 Volume.32 No. 2 p.148 ~ p.154
|
|
|
Validation of Simultaneous Analysis Method of Standard Compounds inFermented Kalopanax pictus Nakai by Bioconversion
|
|
Jang Won-Hui
Lee Wha-Young
Lee Bong-Jin
Kim Jean-Man
Park Seon-Ju
|
|
|
Abstract
|
|
|
|
The aim of this study was to select compounds for the standardization of fermented Kalopanax pictus Nakai (KP-F), to develop the analysis method using HPLC-PDA and to perform method validation. KP-F is a fermented powder developed to improve the original physiological activities and create a new functionality. Eleutheroside E, Acanthoside B, and Syringaresinol were selected as the standard compounds and developed our own method for simultaneous analysis. The analyte was isolated using C18 column with a gradient elution of 0.05 M phosphoric acid in water and methanol as the mobile phase at a flow rate of 1 mL/min and detected at 210 nm. As a result, all standard compounds showed good linearity with an R2 (coefficient of correlation) of 1.000 and for the limit of detection range of 0.710~0.831 μg/mL, and the limit of quantification as 2.150~2.520 μg/mL. The precision was RSD (%) of less than 4.80%, while the accuracy was 4.70%>RSD (%) for the range 102.44~110.48%. In conclusion, the developed analysis method is suitable for the detection of Eleutheroside E, Acanthoside B, and Syringaresinol in KP-F.
|
|
|
KEYWORD
|
|
|
Kalopanax pictus, eleutheroside E, acanthoside B, syringaresinol, method validation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|